Functional Proteomics for biomarker characterisation

Discovery of biomarkers has advanced significantly in the past few years due to the new developments in Genomics, Proteomics, Metabolomics and other systematic approaches. However, one area of systematic biological analysis that is still relatively underserved is the rapid elucidation of the biological functions of a proteome.
Let’s take a step back. Proteins are present in the cell, but they also have a function. Therefore, in some instances, the mere identification of the presence of a protein may not be enough to elucidate the biological pathways involved in a given disease. It is for this that activity assays are needed.

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Host Cell Protein Analysis in Biologics

In a previous post, we looked at innovative methods to assess immunogenicity in biotherapeutics and biosimilars.

The objective though, is to have the least immunogenicity possible (exception made for vaccines). Some factors, especially those which are patient-related, cannot be always controlled but some can definitely be monitored during the production processes of biotherapeutics and biosimilars. Let’s take the case of  Host Cell Proteins (HCPs).

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Pooling samples for proteomics – biomarker profiling case studies

To pool or not to pool biological samples? This question might pop up in the mind of anyone designing biomarker discovery approaches!

Much debate has been raised on biomarker discovery from clinical cohort studies, since the first experiments linking SNPs to disease phenotypes, to the current and new proteomic and miRNA technologies. The answer to this question strictly depends on clinical data, patient group characteristics and… financial means.

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Secretome biomarker identification: tech tips for selecting the most suitable tools

In a previous post, I mentioned that the secretome is becoming a very hot topic in the world of proteome analysis, and even a crucial study subject. Today, I’d like go a step further and shed some light on how to analyse it.

Let’s see what the options are…

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Proteome, secretome, kinome… What’s it all about?

Proteome is referenced as the result of expression of the whole genome. With the recent developments of genomics, one could consider that this analysis is enough to decipher any biological mechanism…
But depending on your sample, some regulations occur, that are not directly linked to the genes: isoform splicing, post-translational modifications (such as phosphorylation, acetylation & glycosylation), secretion…

Genomics, although powerful, does not allow the analysis of such protein-specific modifications. To overcome these limits, proteomics emerges, with specific subsets having specific requirements. Here, I’d like to put the Secretome and Kinome in the highlight!

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