6 tips for thawing hepatocytes

Cryopreserved hepatocytes contain the major hepatic drug-metabolizing enzymes required to study the four categories of xenobiotic biotransformation: hydrolysis, reduction, oxidation and conjugation. Cryopreserved hepatocytes are ready when you are. A simply quick-thaw protocol is performed to remove cryoprotectant and have viable cells… However, the functionality and the viability of these cells can be impaired by an incorrect thawing procedure… [Read more…]

Activation & de-activation pathways of hepatic fibrosis

Liver fibrosis is the result of an imbalance between production and dissolution of extracellular matrix. It has been described that Stellate cells, liver myofibroblasts, and bone marrow derived cells converge in a complex interaction with hepatocytes and immune cells to induce response to liver injury.

[Read more…]