Cytokine signature in airway inflammation with arrays

Chronic Mucosal Inflammation is the hallmark of common airway diseases (ex.  Allergic Rhinitis and asthma). Lipoxin B4(LXB4) is an endogenous mucosal protective mediator decreasing such diseases. LXB4 mechanisms of action remain poorly understood.

Cytokine Quantibody Arrays from Raybiotech and tebu-bio laboratories

Cytokine Quantibody Arrays from Raybiotech and tebu-bio laboratories.

In a recent paper in Mucosal Immunology (Karra, L. et al. (2015) 8; DOI:10.1038/mi.2014.116), Allergic Rhinitis  and asthma murine models have been described to better investigate the role of LXB4 in Mucosal Inflammation. The authors conclude that, in the upper airway, LXB4 significantly decreases nasal mucosal leukocytes and degranulation of Mast Cells (MCs) and Eosinophils. They also show that, in the lower airway, LXB4 significantly decreased airway inflammation, mucus metaplasia, and hyper-responsiveness.

Inhibition of MC degranulation in vivo by LXB4 is more potent than Dexamethasone (a well-known apoptosis inducing glucocorticoid) with unique profiles for cytokine regulation. This latter  is proved by  quantitative analysis of 20 murine cytokines in a single array (Quantibody Cytokine Array).

These findings indicate that LXB4 carries cell type selective and mucosal protective actions and need to be translated to human models. This publication opens the way to consider lipoxins as new therapeutical candidates.

tebu-bio: European RaybioTech's Certified Laboratory service provider

tebu-bio laboratories are the European RaybioTech’s Certified Laboratory service provider.

The Cytokine Arrays are available as ready-to-use assays. They can also be outsourced to Certified Service Laboratory Providers as fee-for-services. In that case, researchers just send their samples and in return receive the experimental data set. To ensure top quality in the data obtained, Raybiotech’s service providers successfully complete training and certification programs to receive the “RayBiotech Certified Array Service Providers” yellow award.

In Europe, tebu-bio laboratories (France) were among the first laboratories in the world to be certified by Raybiotech in 2012. tebu-bio’s services also cover Quansys BioSciences and FullMoon BioSystems technologies for outsourcing protein profiling and quantification.

Invasion of influenza A

The influenza season in Europe is over… until next year. Influenza remains one of the most important public healthcare problems, especially in risk groups.

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Figure 1. Influenza A life cycle, indicating target molecules for which specific antibodies have been developed.Influenza virus can cause annual epidemics of approximately 500,000 deaths per year worlwide, which is facilitated by the high mutation rate of the viral genome and polymerase. The function interplay between hemagglutinin and neuraminidase glycoprotein characterises the pathogenic profile of each viral subtype (1).

The Influenza A virus (IAV), mimicked as misfolded protein aggregates, carries unanchored ubiquitin chains to exploit host cell’s aggresome processing machinery for efficient IAV entry and capsid dissociation (2).

For studies involving IAV, it is important that antibodies recognise the specific viral subtype, with high specificity, to avoid any cross-reactions with other subtypes that may not be relevant that season. [Read more…]

Circulating biomarkers – there’s more than human

In a previous post, we discussed about how important it is to have research tools that allow to study secretome biomarkers outside the usual human and rodents. Either because other species are important research models, or more importantly, because they are the final patient that will be treated, such as pets or cattle.

miRNAs are key mediators in cell processes, allowing to activate or inhibit the expression of certain genes important in different physiological processes. How miRNAs work, and which ones are relevant, are a hot topic of research nowadays, and important advances are being made in areas such as Oncology and the Immune response. [Read more…]

Innovation in Life Sciences: Bead Arrays for Antibody and Complement Profiling by Ayogly et al.

Ayogly et al. have designed an innovative suspension bead-array experimental approach for studying in one assay both antigen-specific antibody reactivity and complement activation induced C3 deposition.

The benefits of such a scalable approach is based on its capacity to measure multiplex antigen-specific complement activation in the context of an autoimmune disease (ex. Rheumatoid Arthritis) and viral antigen detection (ex. Epstein-Barr virus encoded Nuclear Antigen-1 (EBNA-1)).

The combination of antibody reactivity profiling and induced complement activation in one assay open new perspectives in autoantibody- and virus-induced immune diseases.

Want to know more?

Ayoglu B. et al. “Bead Arrays for Antibody and Complement Profiling Reveal Joint Contribution of Antibody Isotypes to C3 Deposition (5 May, 2014) PLoS ONE, CrossRef DOI Link to Publisher-Maintained copy: http://dx.doi.org/10.1371/journal.pone.0096403

Congratulations to the authors, who turned to tebu-bio’s range of products when selecting their antigens (ex. EBNA-1 protein) coupled to beads to determine the optimal assay conditions for measuring complement activation.

Immuno B4B-Connection | April 16-17, 2014

Meet Nathalie Bervas, our “antibodies & arrays” specialist  at the Immuno B4B-Connection.

[Read more…]