Keen to work with the best quality nucleosomes?

Histone Structure- tebu-bio

Fig. 1: Structure of a mono-nucleosome.

Nucleosomes are basis units of DNA packaging in eukaryotic cells. A core particle (mono-nucleosome) consists of a segment of DNA called core DNA (147 bp in length) wound around a histone octamer (Fig 1.). Histone octamers are made up of 2 copies of the the core histones H2A, H2B, H3, and H4. These mono-nucleosomes are connected by linker 80bp-long DNA. H1 histone, the so-called linker histone, binds to the linker DNA close to the entry and exit of the core DNA and is involved in chromatin compaction (Fig 1.). [Read more…]

Site-specific modified-histone substrates

Recombinant Histone proteins are popular research solutions in Drug discovery and Epigenetics studies. They can be used as in vitro positive controls in the analysis of post-translational Histone modifications, as substrates for Histone modification enzymes and chromatin studies.

[Read more…]

5 new research tools to perform chromatin and epigenetic analysis

Nucleosomes are core particles involved in DNA packaging in eukaryotic cells. They affect gene expression levels  by regulating access of DNA to transcription factors and DNA binding proteins. Nucleosome modeling is not static and chaotic. The link between nucleosome dynamics and gene expression modulation still remains a mystery, driving Life Scientists to design clever nucleosome-based in vitro assays such as those we’ll be reviewing here.

[Read more…]

Histone Peptide Arrays’ contribution in Epigenetic studies

EpiGold™ Histone Peptide Array Data-FluorescenceDNA methylation and histone post-translational modifications are key epigenetic factors.

They regulate gene expression by interfering with the chromatin structure and the binding of effector proteins to post-translational modified histones (1).

Several experimental strategies to analyze such binding have recently been designed; histone modified peptide arrays being one of them. [Read more…]