Column-free, Caustic Solvent-free DNA or RNA Purification

Epicentre® (an Illumina Company) has developed a series of MasterPure™ DNA and RNA purification kits for nearly any sample type. While Epicentre’s QuickExtract™ DNA Extraction Solution is designed for the fastest extraction of low abundance DNA for PCR, the MasterPure™ kits yield the highest quality DNA and RNA for applications such as:

  • NGS genomic DNA and RNA-Seq library preps
  • DNA methylation/epigenomics studies
  • Genomic DNA and cDNA cloning
  • qPCR and qRT-PCR

These kits offer a simple protocol for the purification of Genomic DNA, cellular RNA, or total nucleic acid (TNA):

DNA RNA or Both

[Read more…]

Buccal cells: best surrogate tissue for brain DNA methylation studies

A recent report published in the American Journal of Medical Genetics (Smith et al. 2014) describes a methodical comparison of buccal cells and blood as potential surrogates for brain tissue in epigenomic studies aimed at assessing the methylation status of DNA. The methylation patterns of specific genes are thought to be biomarkers for a variety of psychological disorders and may be the result of such factors such as childhood abuse, malnutrition, and traumatic stress. The authors find that DNA methylation patterns in buccal cells are more similar than blood to those of brain tissue. Certainly it is an attractive notion that permanent changes in the methylation patterns of the genetic code are at least partially responsible for some chronic psychiatric and behavioral traits, but the idea that a simple cheek swab can help unravel these mysteries even better than a blood sample is the part we find the most fascinating. [Read more…]