Column-free, Caustic Solvent-free DNA or RNA Purification

Epicentre® (an Illumina Company) has developed a series of MasterPure™ DNA and RNA purification kits for nearly any sample type. While Epicentre’s QuickExtract™ DNA Extraction Solution is designed for the fastest extraction of low abundance DNA for PCR, the MasterPure™ kits yield the highest quality DNA and RNA for applications such as:

  • NGS genomic DNA and RNA-Seq library preps
  • DNA methylation/epigenomics studies
  • Genomic DNA and cDNA cloning
  • qPCR and qRT-PCR

These kits offer a simple protocol for the purification of Genomic DNA, cellular RNA, or total nucleic acid (TNA):

DNA RNA or Both

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Alternatives to the “mega-brands” of molecular biology

If you don’t buy luxury brand clothes, why are you still buying luxury brand molecular biology kits?

Let me guess… you might use those blue and red boxed Qiagen® kits for DNA and RNA purification, maybe TRIzol® if you’re a real rebel. You probably also use a SuperScript® RT Kit for reverse transcription and you can’t keep track of which company most recently bought Invitrogen® to control that logo that you’re loyal to.

Maybe it used to be the case that these were the only companies making these kits, so you really had no choice. Now, many of these products are essentially commodities, but you’re still used to purchasing the “first to market” brand because it works and it would probably cause trouble for your lab to change brands. The perceived “switching costs” are large part of the reason for this persistent brand loyalty, and part of the reason why ThermoFisher was willing to pay $13.6 billion for the Life Technologies brand in 2013. There is an entire arm of corporate finance devoted to assigning the value to a brand. Essentially, it tries to estimate how much more customers are willing to pay for a commodity item just because of the company logo on the side of the package.

Here, we examine some alternatives to 2 of the biggest molecular biology mega-brands that seem to be able to keep their loyal customers by placing their familiar brand name on a relatively low value item:

1 – Alternatives to Lipofectamine® Reagents

There’s the one with the blue cap, the one with the red cap, and the one with the green cap, and most people don’t really understand the difference, but cell biologists seem to love these transfection reagents and are willing to pay a heavy premium for them.
Compared to the prices announced in 2015 on the Life Tech website for the 1.5 mL size Lipofectamine® 2000, similar products from competitors are available for about 1/4 the price:

LipoD293 DNA (Ver. II)  1 ml

– Enhanced conjugate with liposome for maximal efficiency
– Best for lentivirus, antibody and protein production

GenMute™ siRNA  1 ml

– Exceptionally good for siRNA transfection
– Excellent for DNA/siRNA co-transfection

LipoJet™ DNA  1 ml

– Fluorinated cationic lipids
– Best transfection efficiency with least toxicity

Validation data for Lipojet™, for example, suggest that it is superior to many other commercial transfection reagents including “L2K”::

Lipojet Multiple Cell Line Data

Lipojet Multiple Cell Line Data

LipoJet_L2K_PolyJet_293T

Given the vast selection of transfection reagents available today, the best option is usually to contact a technical specialist (at tebu-bio for example) for advice on the most suitable ones for your research.

2 – Alternatives to Qiagen® and TRIzol®

Whether you’re using the DNeasy® and RNeasy® kits or TRIzol® products for DNA and RNA extraction, chances are you just keep buying the same kit you learned to use when you were a student. Sure, there are companies making less expensive column-based kits, and there are even protocols online explaining how to regenerate the used columns and make your own buffers. You can also find generic RNAzol RT RNA Isolation Reagent. 

The advances we really like, however, are the purification systems that work without columns and without dangerous solvents. Here are a few of our favourites:

QuickExtract™ DNA Extraction Solution The fastest, least expensive option when you just need to purify DNA for PCR.

QuickExtract™ RNA Extraction Kit  Available in two sizes: the extremely competitively priced 5mL kit contains sufficient material to perform 50 RNA extractions, while the 50mL kit contains sufficient material to perform 500 RNA extractions.

MasterPure™ DNA and RNA Purification Kits  These are for the times when you need the highest quality nucleic acid for next generation sequencing, qPCR, or PCR. Produced by the RNA biology experts at Epicentre (an Illumina company), MasterPure™ Kits are available for nearly every application and source including mammalian cells, yeast, blood, Gram Positive bacteria, and Plant Leaves.

As is the case with transfection reagents, choosing a new nucleic acid purification kit is not always easy, so feel free to contact a technical specialist at tebu-bio for advice.

To get in touch, just leave your comments or questions below.

 

DNeasy® and RNeasy®, and Qiagen® are registered trademarks of the Qiagen group. TRIzol® is a registered trademark of Molecular Research Center, Inc. Invitrogen® and SuperScript® are registered trademarks of Invitrogen. Lipofectamine® is a registered trademark of Life Technologies, Inc. 

Buccal swab DNA Collection with no cold chain required

For years tebu-bio has been selling Epicentre-Illumina’s BuccalAmp™ system for non-invasive DNA sampling from inner cheek buccal cells. For many research studies, we find that researchers need two important things from a swab-based DNA collection system. First, the system must yield high quality DNA suitable for Next Generation Sequencing applications. Secondly, the system must instantly stabilize the sample to allow for at-home or remote location sampling (no cold chain required). In this post, I will introduce you to the iSWAB™-DNA Collection Kits.

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