Unraveling the protective role of the stroma

Research performed by David García-Molleví and his team at ICO (Barcelona, Spain), in cooperation with tebu-11018152_928445027176257_6580852836315207360_nbio laboratories, is being presented at the AACR congress in Philadelphia these days.

The poster describes how cytokine profiling of drug-disrupted tumour cell / fibroblast crosstalk provides insights to understand the protective role of the stroma. Briefly, an array including 174 cytokines was performed on tumour cell / fibroblast co-cultures. Results determine that IL1b and TGFb1 secreted by tumor cells trigger the activation of normal colonic fibroblasts (NCF) to become CAFs. The role of IL1b is not as well known as TGFb1 in a cancer context.

Cytokine arrays were used in order to determine:

i) cytokine profiling of IL1b-treated NCF
ii) profiling of tumor cell-NCF cocultures in the presence of inhibitors of IL1b and TGFb1 signaling, main triggers of NCFactivation.

This poster shows how cytokine profiling can be useful as a complementary approach for microenvironment studies in assessing reciprocal activation of tumour cells and stroma, mediators of such interplay, treatment effectiveness and new target interventions.

Would you like to have a copy of this poster? Contact us!

New biomarkers in periodontal disease

Periodontitis, also generally called gum disease or periodontal disease, begins with bacterial growth in the mouth and may end, if not properly treated, with tooth loss due to destruction of the tissue that surrounds your teeth. It’s not only an issue of aesthetics, it’s also an issue of health, as it might affect nutrient intake and digestion, affecting quality of life.

To make it simple, depending on the seriousness of the disease, we talk about either gingivitis (gum inflammation), that usually precedes periodontitis (gum disease). Not all gingivitis progresses to periodontitis. [Read more…]

Biomarker profiling: Diluting samples case studies (II)

Back to dilution of samples. After our post on whether samples should be diluted or not, let’s discuss today about how much they should be diluted.

Case # 1 – ELISA or simplex assays

Most ELISA manufacturers indicate the dilution range for different types of samples (serum, plasma, cell culture supernatants, etc.) to be used in their products. However, the dilution range can sometimes be from 1:2 to 1:2,000. How to choose the right dilution factor?

The honest answer is: nobody knows. That is,  nobody knows what is the right dilution factor in your specific research project, for your specific biomarker. [Read more…]

Diluting samples for proteomics – biomarker profiling case studies (I)

To dilute or not to dilute biological samples? And if yes, how much? That’s the question!

Following our post on whether samples should be pooled or not when performing studies for Biomarker discovery, it’s now time to discuss sample dilution (yes or no) and dilution factor (how much) when performing proteomic analysis of Biomarkers. [Read more…]

Antibody arrays: a matter of species

Research is never easy. That’s why we either like doing it or supporting it.

That said, it’s easier for some researchers than others. If you are working on human, mouse or even rat models, you have quite a wide variety of tools available. Even if you work on zebrafish, at least, you have some validated zebra antibodies, guaranteed to work for your animal model.

What if you are working on other “exotic” animal models? Not by choice, but because they are really the relevant models for your research. Let’s take some examples. [Read more…]

Adipokines in obesity and glucose homeostasis

Obesity is the abnormal increase in adipose tissue mass. This phenomenon increases the likelyhood of a number of diseases such as heart disease, high blood pressure, type 2 diabetes, obstructive sleep apnea, osteoarthritis, and some kinds of cancer.

Obesity is probably the leading preventable cause of death worldwide. People are considered to be obese if their body mass index (BMI) exceeds a value of 30. The mainstays of treatment of obesity remain dieting and physical exercise.

Nevertheless, adipose tissue (fat) cannot be considered a worthless batch of “lard”, but rather a fascinating vital tissue that in addition to being the body’s major energy reservoir, plays a central role as a secretory organ. Research in this field has already lead to exciting results related to both fat reduction, insulin resistance and to the development of robust tools to study diabetes / obesity.

Adipokines are factors secreted by adipose tissue which carry messages to other parts of the body. I would like to briefly introduce the role of adipokines in the regulation of body weight and glucose homeostasis. [Read more…]

Secretome biomarker identification: tech tips for selecting the most suitable tools

In a previous post, I mentioned that the secretome is becoming a very hot topic in the world of proteome analysis, and even a crucial study subject. Today, I’d like go a step further and shed some light on how to analyse it.

Let’s see what the options are…

[Read more…]