8 criteria for selecting your ELISA kits

Biomarkers specialists are often asked to select an ELISA kit for researchers: with thousands of ELISA references available on the market, the choice can be tricky regarding proteins for which several kits available.

When researchers have to choose a new ELISA kit, the price is regularly the first parameter of selection. But my experience with long term projects shows that it should in fact be the very last one…

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Biomarker profiling or multiplex quantification for everyone

Many researchers would be keen to identify new targets for their research project: add a new cytokine to the classical inflammatory panels, find the missing link between 2 phosphorylation pathways, dig into the miRNA to find a new therapeutic target…
They expect they’ll need dedicated (and expensive) new equipment. Not necessarily! Let’s take a look at assays that use existing and quite common readers, or that can easily be outsourced to reliable labs…

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Secretome biomarker identification: tech tips for selecting the most suitable tools

In a previous post, I mentioned that the secretome is becoming a very hot topic in the world of proteome analysis, and even a crucial study subject. Today, I’d like go a step further and shed some light on how to analyse it.

Let’s see what the options are…

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Proteome, secretome, kinome… What’s it all about?

Proteome is referenced as the result of expression of the whole genome. With the recent developments of genomics, one could consider that this analysis is enough to decipher any biological mechanism…
But depending on your sample, some regulations occur, that are not directly linked to the genes: isoform splicing, post-translational modifications (such as phosphorylation, acetylation & glycosylation), secretion…

Genomics, although powerful, does not allow the analysis of such protein-specific modifications. To overcome these limits, proteomics emerges, with specific subsets having specific requirements. Here, I’d like to put the Secretome and Kinome in the highlight!

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