Innovative solutions in cosmetology: Cosmetic 360

The world’s leading perfumery cosmetics network, Cosmetic Valley, announces that its 2015 Cosmetic-360 Meeting will take place in Paris (October 15 – 16th, 2015).tebu-bio belong to the Cosmetic Valley Network.jpg

As a member of this network, tebu-bio will be participating in this trade show which is focussed on open innovations this year.

Recent advances in R&D in vitro cosmetic testings

Several innovative solutions have been designed by tebu-bio laboratories to better understand the mechanisms of action of ingredients, and also to further characterize efficient compounds.

These solutions cover cell-based and functional assays but also gene expression analysis.

Cell culture platform

Starting with cell culture, tebu-bio benefits from a huge bank of primary human cells (including keratinocytes, fibroblasts, adipocytes, sebocytes…) from various sources (donors with a large range of choice ex. gender, age, type, body zones) to develop tailored experimental projects.

Cosmetic-360, Paris 2015As an example, using keratinocytes from young vs. aged donors gives the opportunity to identify biomarkers involved in senescence and by comparison with those present in young donors, determine pathway and efficiency of active compounds. These projects do not need any artificial aging tools like UV irradiation which can generate pathway specific aging process and are not always representative of all natural mechanisms involved. Another approach uses adipocytes issued from normal or high BMI donors, which are helpful tools for testing all slimming mechanisms, including the adipokine pathway.

In combination with oxygen controlled cell culture devices, tebu-bio offers a wide variety of cell culture models including strict physioxic cell conditions for more predictive results and by modifying oxygen concentration they are able to create oxydative stress assays as well as hypoxic stress environment.

Profiling approaches

After cell culture treatment, tebu-bio’s labs offer unique access to a wide variety of biomarker identification tools (from cell culture supernatants for soluble released signaling biomarkers or intracellular biomarkers impacting cell behaviour). Approaches are multiple to allow high versatility such as :

  • Pathway specific gene profiling arrays (oxydative stress, senescence, innate immunity, inflammation, cell metabolism etc…)
  • Protein profiling arrays for qualitative (like multiple Western Blots with up to 1,350 markers per array) or quantitative (like multiple ELISAs with up to 360 markers at once) approaches. Markers available are soluble ones which will impact cell environment or intracellular ones wich will modify internal cell behaviour.
  • miRNA profiling – highly promising biomarkers are either secreted and found in cell culture supernatant or remain intracellular. miRNAs have the capacity to down regulate RNA translation and are thus powerful mediators of cell activity.

Bioassays

To further complete the analysis of active compounds, functional bioassays are ideal and available as ready-to-use kits or as fee-for-services. The most popular ones include:

Come and meet us at Cosmetic 360 – get your free invitation

I will be pleased to meet you at Cosmetic 360 and discuss the recent advances in cosmetology and in R&D. To receive an invitation to Cosmetic 360 free-of-charge offered by tebu-bio, get in contact via the form below. The number of complimentary inviations available is limited, early application is advised.

Anti cellulite compound evaluation with in vitro adipocyte-assays

Mechanisms leading to cellulite formation is complex. It involves lipid regulatory pathways and proinflammatory cross-talk that represent promising molecular targets in cosmetology. This post introduces a clever in vitro adipocyte-based assay targeting adipocytokines to better determine the anti cellulite effects of cosmetics compounds.

[Read more…]

5 reasons to prefer custom rec. protein production to commercial

Many recombinant proteins (rec. proteins) are now commercially available. And many of them, mainly secreted soluble proteins, are biologically active, so there shouldn’t be any need to look for alternatives such as custom productions.

So the question is, why should you prefer a special production?

Well, there are several reasons (and they’re good ones too!). [Read more…]

Direct access to Biomarker Profiling identification tools

Profiling tools have an increasing interest for identifying new biomarkers. Different tools are available, from classical gene profiling on DNA chips, RT-qPCR arrays, Protein arrays for secretome or transcriptome, mRNA arrays and finally miRNA arrays. All these techniques sometimes require specific material for reading arrays, and some bioanalytics to extract valuable markers of interest.

Expand your knowledge – a new visionArrays - Blog Thumbnail

The increasing focus on these tools is obvious as they help to expand scientific knowledge on sometimes well-known pathways. Why restrict your analysis to classical markers instead of checking the impact on more than 100 biomarkers… at a similar cost? Value of results is obvious as well, as it offers a convenient way to identify original pathways.

The “Start smart” attitude

Facing a new project is always a stressful situation. Biomarkers chosen are generally based on literature, which may lead to duplicating, more or less, already existing information. Searching for innovative pathways looks more like a cherry-picking, highly risky strategy. At the end, this may lead to rather conservative conclusions. A striking example concerns Western blots (WB) which everybody knows may be time consuming, rather expensive and for sure limited in number for a given project. Nobody will ever make the decision to perform more than 100 WB to explore all the potential targets available. Lab’s budgets can’t survive such a strategy, and probably the time allocated to a project cannot suffer such delays…

Protein profiling on secretome or transcriptome now allows you to study more than 1000  targets from a single sample at once. Pricing is equivalent to 10 to 20 WB traditional WB. Results obtained are of top value as they immediately orientate research focus to appropriate pathways without having the risk of missing crucial information. This helps to speed up projects, focus research towards original biomarkers and at the end deeply differentiate published results.

But not everybody has the appropriate material to perform these assays, nor is used to handling these arrays. This generally needs adapted scanners to perform readouts and once results are obtained, spots need to be further analyzed to guarantee final quality. It requires some skill. One can understand the reluctance to jump into these technologies when they are only of occasional need.

So where’s the solution?

For these reasons, tebu-bio has developed over these last years a complete Profiling – Biomarker identification platform that offers researchers access to various solutions. With a complete offer including RT-qPCR arrays, protein arrays for soluble or signal transduction markers, mRNA and miRNA arrays, we help researchers to immediately identify biomarkers involved in their domains. This information, which often appears as the initial step in project management, drives studies in the appropriate direction, rapidly and in a cost effective way.

The service process is extremely simple. After defining the most appropriate solution regarding goals of interest, your samples are tested and results sent back. Time frame is generally 2 to 4 weeks depending on starting material. A dedicated project manager is always available for any questions throughout the project. There is no license involved in these studies, which are performed in our own, European-based labs near Paris.

For those who don’t have access to cell culture facilities, our cell culture platform is also ready to collaborate, with access to a large stock of primary cells.

And the next step…

Well, if you’re curious to know how this could help boost your research, get in touch to see exactly how it can work! Just leave your question or comments below.

Why not outsource your plasmid preps ?

Plasmid preps are to science what salt is to cooking. It’s so common that nobody notices, but nothing can be done without. There is no mention of this step in any published paper, nobody mentions them in any activity report, no comments are made on time spent on it.Screen Shot 2014-12-31 at 4.55.17 PM

But let’s face it: it takes time, as simple and as usual that it is. Some days spent, here and there. It’s so well planned in your weekly agenda, it’s such a habit, that you don’t even think about it. It’s just routine. When you’ve done a plasmid prep a couple of times, there’s nothing new to learn about it. It’s like riding a bike, once you know you don’t even notice any more.

But it takes time.

[Read more…]

Physioxia or Hypoxia: what it’s all about

Cell culture under oxygen controlled conditions: an improvement towards more predictive results

Oxygen concentration in tissues is a key factor for cell and organ survival. In normal conditions, partial oxygen pressure (pO2) results in the balance between oxygen delivery and its consumption. Oxygen is transported, in mammals, by circulating red blood cells. Partial oxygen pressure in tissues varies widely, depending on their respective metabolic requirements and their functional status. In normal physiological conditions,  partial oxygen pressure is called physioxia. Any alteration of tissue environment leading to a decrease in partial oxygen pressure is called hypoxia. Hypoxic conditions have been observed in many different pathological situations like tumor development, obesity or transcient ischemia. [Read more…]