Actin and Tubulin dynamics research studies in 2015

The world of cytoskeletal dynamics studies is in mutation. By example, the first synthetic chemical targeting actin and specifically triggering its growth has been recently released for research applications (BPA). A few weeks before, SiR stains were successfully launched for Actin and Tubulin Live Cell Imaging (SiR-actin Live cell Imaging was at the JBC frontcover in Feb. 2015).

Cellular and molecular biologists have now access to a unique range of discovery tools opening new perspectives for deciphering cytoskeletal events living cells.

Branched PolyAmines (BPA)

Until now, only molecules that stabilize or destroy the cytoskeleton of actin were available. Derived from supramolecular chemistry, Branched PolyAmines (BPA) rapidly enhances the growth of lamellar networks of actin filaments.

BPA-induced actin filaments

Actin filamentous network growth 10 minutes after addition of BPA to cell cultures.Source: Pr. Daniel Riveline, Laboratory of Cell Physics, ISIS/IGBMC, Strasbourg (France).

BPA is a specific modulator for in vitro and in vivo actin dynamics studies by regulating actin nucleation and turnover in cells. This compound complements currently available molecules to study actin dynamics such as Latrunculin A , Cytochalasin D, Jasplakinolide, WiskostatinActin Binding Proteins.

Transfection-free SiR Actin and Tubulin stains

SpiroChrome’s live cell SiR actin stains are non-toxic fluorescent stains for monitoring Actin and Tubulin changes in living cells. These cell permeable SiR-Actin and SiR-Tubulin compounds stain microtubules and F-actin respectively in living cells.

SiR Tubulin tebu-bio's fluorescent dye

3D-SIM microscopy image of labeled microtubules in primary rat cortex neuron body stained with SiR Tubulin.

Novel research tools aiming at better understanding cytoskeletal reorganization have radically changed discovery frontiers. Interestingly, and despite their degree of innovation, these new reagents remain very affordable and user friendly for research applications.

tebu-bio’s experts will follow on tracking emerging cystoskeleton-based technologies.

 

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