Focus on the Calcineurin-NFAT Pathway…

A few weeks ago, I received an email from one of our blog readers. Following my post about the Autophagy pathway, she asked me for the same kind of post about the Calcineurin-NFAT Pathway (Cn-NFAT). With my colleague, Ana (thank you!), we did some research about this pathway and this post is the result. We hope it will help you!

The distinguishing feature of the Cn-NFAT pathway is its regulation by Ca2+ and the Ca2+/calmodulin-dependent serine phosphatase calcineurin. NFAT proteins are phosphorylated and reside in the cytoplasm in resting cells; upon stimulation, they are dephosphorylated by calcineurin, translocate to the nucleus, and become transcriptionally active, thus providing a direct link between intracellular Ca2+ signaling and gene expression.

NFAT activity is further modulated by additional inputs from diverse signaling pathways, which affect NFAT kinases and nuclear partner proteins.

The Cn-NFAT pathway seems to activate transcription of a large number of genes during an effective immune response, including the activation of distinct subsets of target genes in lymphocytes, as well as participation in an early phase of chromatin remodeling that occurs at specific genetic loci in differentiating T cells (1).

The Cn-NFAT pathway also has a role in vertebrate organogenesis and development too (2).

Schematic view of the NFAT activation cycle

Schematic view of the NFAT activation cycle

tebu-bio has selected a panel of primary antibodies related to this pathway

  • Anti NFATC1:  a rabbit polyclonal to NFATc1
  • Anti NFATC2: a mouse polyclonal antibody H00004775-B01P-4-1-1-S
  • Anti NFATC3: a mouse polyclonal antibody raised against a full-length human NFATC3 protein.
  • Anti FOS Human: Mouse monoclonal antibody raised against a partial recombinant FOS.
  • Anti Jun: a Rabbit polyclonal to c – Jun

Looking for a specific intracellular pathway?

We strive to provide you regularly with new and updated pathways to support you in your research. If you are looking for a specific pathway, don’t hesitate to contact us.

Sources

(1) Transcriptional regulation by calcium, calcineurin, and NFAT Patrick G. HoganLin ChenJulie Nardone, and Anjana Rao

(2) http://crablab.stanford.edu/labinterestnfat.html

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