Your neurons will forget Lipofectamine!

Mature cultures of neurons and other neural cells are extremely valuable for in vitro neurotoxicity studies and screening for agents that can slow, stop, or even reverse the course of neurodegenerative diseases.

The transfection of nucleic acids into neurons is essential for studying many aspects of neurobiology. However, neurons are among the most difficult cell types to transfect. They are very sensitive to culture conditions, presenting a particular challenge with regards to efficiencies. In addition to yielding low efficiencies, currently available cationic lipid reagents are often toxic to the cells, compromising post-transfection experimental results. While some viral mediated gene delivery systems have been shown to produce high efficiencies, they are very labor intensive and inconvenient for most researchers, along with the inherent danger and risk of provoking an immune response in the cell and/or interfering with the host genome. So what other solutions exist? This recently introduced alternative is well worth discovering…

Neurons-DNAIn

About DNA-In Neuron

Developed by the co-inventors of the early Lipofectamine products, DNA-In™ Neuro was formulated from a novel chemistry for maximum transfection efficiency in neurons. In side-by-side assays with top competitor reagents significantly higher transfection efficiencies are consistently observed when DNA-In™ Neuro is used to transfect primary cortical, hippocampal or forebrain neurons. Moreover, with low cytotoxic effect DNA-In™ Neuro supports neuronal survival and neurite extension post-transfection.

Feature Benefits:

  • Higher Transfection Efficiency – Two-fold or greater improvement in efficiency over competing transfection reagents
  • Exceptionally Low Toxicity – Maximum post-transfection neuron viability critical for performing assays on healthy, uncompromised transfected neurons.
  • Highly Robust Performance – Produces consistent and reproducible results.
  • Quick and Easy-to-Use – An easy to follow, single-tube reagent protocol for great results on the very first try.

Superior transfection efficiency
DNA-In™ Neuro is a new transfection reagent that consistently produces high transfection efficiencies in neurons, typically achieving a 2-fold or better improvement in efficiency over the competing reagents currently available, including Lipofectamine® 2000 and NeuroFECT™. Moreover, DNA-In™ Neuro enables neurons to be efficiently transfected with minimal toxicity to support healthy post-transfected neurons, critical for performing assays on uncompromised transfected cells.

DNA-In_Neuro_vs_L2K

Improved_Efficiency_in_Neurons

Figure 1 (Top,Bottom). DNA-In Neuro Outperforms Leading Competitor ReagentDNA-In™ Neuro was used to transfect plasmid DNA encoding GFP into 6-day cultured E18 Primary Rat Cortical Neurons. Transfections were performed in 24-well plates using 1.0-3.0µl of DNA-In™ Neuro Reagent. The above data show DNA-In™ Neuro significantly outperforms Lipofectamine® 2000 with near 3-fold improvement in transfection efficiency after 24 hours (Bottom Graph). Duplicate wells were assayed 24-hrs and 48-hrs after transfection.

DNA-In_Neuro_GFP-20xDNA-In_Neuro_GFP-40x

Figure 2. High GFP Expression in Primary Neurons – Primary rat cortical neurons were transfected with DNA-In™ Neuro Transfection Reagent and incubated overnight in complete culture media. The above images were taken 48-hours post-transfection and show uniform, high GFP expression in healthy cells.

ROBUST, EASY TO USE, ONE-TUBE REAGENT

DNA-InNeuro_Protocol_Animation

Related products: Neurons, media & supplement

There’s a variety of both primary and iPS-derived neural cell products that are thoroughly tested for quality assurance that can be used in your neurotoxicity, drug screening or gene expression studies.

Product protocols give step-by-step instruction to achieve maximum post-thaw cell viability and optimal plating densities for your assay, while the serum-free neural media supplements have been optimized and extensive tested to enhance long-term survival and performance.

  • GS21 – a serum-free neural media supplement designed to improve the overall performance of primary neurons in culture.
  • NeuralQ™ Basal Medium – is optimized for maximum growth and long-term viability of primary neural cells in culture

Related readings

Take a look at this post, Induced pluripotent neural stem cells in screening assay, to see the advantages using iPSC cells vs. rodent neurons.

Discover how GS21’s, serum-free Neural Cell Culture Supplement, outstanding properties surpass the B27 component.

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