Adipokine monitoring during Hunger and Satiety balance

Cytokines and growth factors that are produced by or have an effect on adipocytes and related peptide neuro-transmitters are central players in hunger vs. satiety balance and in eating behavior (1). Recent evidence shows that adipokines, and more generally mediators or indicators of inflammation (2), play roles in the development of insulin resistance (3), diabetes (4) and many other concomittant health problems associated with obesity, including hypertension, dyslipidemia and atherosclerosis (5).

This constellation of symptoms are often referred to as “metabolic syndrome”. All of them are associated with abdominal obesity, characterized by extensive subcutaneous and visceral deposition of white adipose tissue (6).

Primary adipocytes or flash frozen floaters by tebu-bio

Primary adipocytes from adipose tissue by tebu-bio.

White adipose tissue is by no means an inert fat-storage depot, but could be better characterized as an endocrine organ, mediating complex signals of inflammation to nearby vital organs (7). Understanding the role of endocrine signaling by white adipose tissue, as mediated by adipokines, is critical to understanding and treating the myriad of diseases resulting from obesity.

Adipokines and cytokines affecting the physiology of adipose tissue are increasingly the focus of translational research projects and clinical studies. Monitoring obesity and other metabolic syndrome biomarkers is now more convenient with new protein profiling and quantification experimental strategies. These antibody-based assays complement the existing arsenal of proteomics tools.

Metabolic syndrome-related protein profiling

Immuno-assays now allow convenient profiling of protein markers involved in obesity from various biological samples (serum, cell culture supernant, body fluids, cell lysate, tissue lysate). The comparison of “protein signatures” between two samples (treated vs. untreated for example) thus becomes much easier. RayBiotech arrays (membrane or glass) allow the detection of 62 adipokines in one experiment (RayBio Sandwich-based Human Obesity Membrane or Glass-Slide Obesity Arrays ), or even up to 182 adipokines (RayBio Label-based Human ObesityMembrane or Glass-Slide Obesity Arrays).

Raybiotech Quantibody Human Obesity array 1 at tebu-bio

Raybiotech Quantibody Human Obesity array 1 at tebu-bio

Obesity Marker quantification by Array or ELISA

Sometimes, a more quantitative approach of the samples is required. New immuno-assays have able been developed to quantify in parallel multiple obesity biomarkers (multiplex quantification) such as the Raybio Quantibody Human Obesity arrays.

As an example, the Quantibody Human Obesity Adipokine array 1 allows multiplex quantification of 10 biomarkers (IGF-I, IL-1beta, IL-6, IL-8, Insulin, Leptin, MCP-1, PAI-1, Resistin, TNF alpha) in one run from only  50 – 100 µl per array (CV<20%).

You might prefer to quantify single Obesity biomarkers through robust ELISA kits (ex. Human Leptin ELISA kitMouse Leptin (Ob) ELISA kit. EIA and ELISA kits compatible with serum, plasma, culture supernantant and cell lysates are also available for other obesity-related targets (ex. AdiponutrinBrain Natriuretic Peptide (BNP)Cholecystokinin (CCK)C-PeptideGastrin EIAGhrelinNeuropeptide YGlucagonPYY…). All these immuno-assasy are validated by specialized manufacturers like Raybiotech, Assay Biotech, BioSensis or Elabscience and compatible now with various species (Human, Mouse, Rat, Sheep…).

Ready-to-use immuno-assays… or a service provider?

tebu-bio: European RaybioTech's Certified Laboratory service providerThese Obesity Immuno-assays are available as ready-to-use kits but they can also be outsourced to Certified Service Laboratory Providers. In that case, researchers just send their samples and in return receive the experimental data set. To ensure top quality in the results given, Raybio’s service providers successfully complete training and certification programs for testing samples with dedicated antibody array products and receive the “RayBiotech Certified Array Service Providers” yellow award. In Europe, tebu-bio laboratories (located in Le Perray-en-Yvelines) were among the first laboratories in the world to be certified by Raybiotech in 2012. tebu-bio’s services also cover Quansys BioSciences and FullMoon BioSystems technologies for outsourcing protein profiling and quantification.

What about you?

Which obesity and metabolic syndrome biomarkers are you following?

References

  1. Murphy & Bloom, Nature. 2006
  2. Fantuzzi, J Allerg Clin Immunol. 2005
  3. Shoelson, et al. J Clin Invest. 2006
  4. Tataranni & Ortega. Diabetes. 2005
  5. Van Gaal, et al. Nature. 2006
  6. Deprés and Lemieux. Nature. 2006
  7. Trujillo & Scherer. Endo Rev. 2006

 

Trackbacks

  1. […] released. This is for example the case for recombinant adipokines (including Leptin) and immuno-assays aiming at profiling or quantifying adipokines.  These latter can even be outsourced to Contract Research Organisations (CROs). In Europe, […]

  2. […] using the protein array technology, proinflammatory adipokines can be easily quantified directly from adipocyte […]

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

%d bloggers like this: