Focus on Lympholyte, the ideal tool to isolate viable lymphocytes!

Lymphocytes (B, T and NK cells) belong to the family of White Blood Cells (WBC or leukocytes) together with monocytes and granulocytes.  Lymphocytes are found in the blood, bone marrow (where they are produced) and lymphoid tissues. They play a central role in the immune system and their blood rate might be affected during infections, genetic diseases, cancers… High quality isolation of lymphocytes is thus a key step in many in vitro research programs covering immunology, infectious diseases and oncology. Here, we review a unique set of Lymphocytes’ separation media called Lympholytes.

CEDARLANE®’s Lympholyte® cell separation centrifugation media has been specifically designed for the isolation of viable lymphocytes from lymphoid organs  by removal of red and dead cells.

For each media, a lympholyte is available.

Lympholyte®-M

Purification of peripheral blood Granulocytes. Rondelli T., Berardi M. et al. PLoS One. DOI: 10.1371/journal.pone.0054046

Purification of peripheral blood Granulocytes. Rondelli T., Berardi M. et al. PLoS One. DOI: 10.1371/journal.pone.0054046

Lympholyte®-M is a density separation medium specifically designed for the isolation of viable lymphocytes from murine lymphoid cell suspensions. It can be used to eliminate erythrocytes, dead cells and debris from murine spleen, lymph node, thymus and bone marrow suspensions.

Lympholyte®-Rat

Lympholyte-Rat is ideal for the isolation of viable lymphocytes from rat lymphoid cell suspensions. Its simple protocol enables the elimination of erythrocytes, dead cells and debris from rat spleen, lymph node, thymus and bone marrow suspensions.

Lympholyte®-H

Lympholyte-H is designed for the isolation of viable lymphocytes and monocytes from human peripheral and cord blood. Used for the elimination of erythrocytes and dead cells from human blood, it can also removes the majority of granulocytes (including neutrophils). The resulting cell population consists of a high and non-selective recovery of viable human lymphocytes and monocytes.

Lympholyte®-Rabbit

Rabbit lymphoid cells suspensions can be cleaned with Lympholyte-Rabbit, thus eliminating erythrocytes, dead cells and debris from rabbit spleen, lymph node and thymus suspensions.

Lympholyte®-Mammal

Lympholyte-Mammal is a density separation medium specifically designed for the isolation of viable lymphocytes and monocytes from the peripheral blood of most mammalian species. It consists of Sodium Diatrizoate combined with Dextran to induce erythrocyte aggregation and reduce platelet aggregation resulting in a higher yield of lymphocytes and monocytes. Lympholyte-Mammal can be used for the elimination of erythrocytes and dead cells from the blood of most mammalian species. Lympholyte-Mammal also removes the majority of granulocytes (including neutrophils).

Lympholyte®-Poly

Lympholyte-poly is a ready-made sterile and endotoxin tested solution which is a mixture of sodium metrizoate and Dextran 500 that allows for the isolation of human polymorphonuclear granulocytes from whole blood. Lympholyte-poly is ideally suited for isolation of polymorphonuclear granulocytes (neutrophils, eosinophils) from human whole blood. Mononuclear and polymorphonuclear leukocytes are separated into two distinct bands free from erythrocytes. It has also been found to be suitable for the isolation of neutrophils from bronchial lung lavage suspension.

Lympholyte®-1.1

Lympholyte-1.1 is specifically designed for the isolation of islet cells from the pancreas. Lympholyte-1.1 can be used undiluted to eliminate non-islet tissue such as exocrine fragments, lymph nodes, ganglia, ductal and vascular tissue from pancreatic cell suspension. The resulting cell population consists mainly of islet cells that may be expanded for use in vivo.

What is your preferred Lympholyte media for your research?

Leave your comments here, and if you’d like some expert advice in identifying and using the best lympholyte, just fire away any questions!

 

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