CRISPR-Cas or TALEN genome editing – which one to choose?

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated)  and Transcription Activator-Like Effector Nuclease (TALEN) are endonuclease based technologies aimed at developing targeted genome editing technologies.

CRISPR and TALEN provide Scientists with unique discovery tools for pathophysiology or genotype-phenotype studies by creating cellular models with gene knock-out, knock-in or tagging, promoter swapping, nucleotide substitution, protein truncation, reading frame disruption, modification of regulation by miRNA, genetic defect corrections…But, which one is the best for your application?

CRISPR-Cas 9 Genome Engineering

CRISPR-Cas 9 representation

Typical TALEN technology

TALEN representation

CRISPR or TALEN?

Which criteria to take into consideration to select TALEN over CRISPR or CRISPR over TALEN?

In a tech note, Ed Davis (Genome Editing expert at GenoCopoeia) compares TALEN and CRISPR tehnologies.

Ed also defines multiple criteria to consider when selecting the most appropriate gene editing method suited to the application of interest (specificity, efficiency, ease of use, methylation sensitivity,off-target effects…).

 

download now your tebu-bio's technical resource

  Download now the  Technical Bulletin “Genome editing: Which should I choose, TALEN or CRISPR” written by Ed Davis!

 

See TALEN and CRISP-Cas9 genome editing technologies in action!

TALENs knockdown eGFP expression GeneCopoeia tebu-bio

(A) eGFP TALENs expression validation by WB. (B) TALENs knockdown eGFP expression by IF in HEK293T cells. Source: GeneCopoeia.

OCT4 and SOX knockin with TALEN in HEK293

(A) OCT4 or SOX2 ORF knockin clones co-transfected with the AAVS1 TALEN pair into HEK293T cells by IF (A) and WB (B). Source: GeneCopoeia.

CRISPR-Cas9 multiplexing to multiple targets

HEK293T GFP-stable cells co-transfected with plasmids expressing Cas9 and multiple sgRNAs targeting p53, HUWE1, NCL3 and GFP (Lanes 1 – 4) or scrambled sgRNA (Lanes 5 – 8) analysed by PCR. Source: GeneCopoeia.

 

 

 

 

 

 

 

 

 

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

%d bloggers like this: