5 tips for successful flow cytometry results

Dr. Camilo Moncada  is Director of QC at Rockland Immunochemicals, a US Biotech leader in antibody developments. With his team of scientists, Dr. Moncada develops and validates innovative antibody-based research tools.

Flow cytometry (FCM) is an attractive experimental approach, especially when accurate monitoring of cell membrane and intracellular pathway signaling biomarkers is required.

In a technical note compiled by Dr. Moncada on tebu-bio’s initiative, he reviews 5 simple but critical experimental steps in order to design optimal FCM protocols and improve flow cytometry results.

Looking for tips and tricks to optimize Flow cytometry results and interpretation?Anti-IL6 Antibody in FCM (cat. nr 209-301-310S by Rockland from tebu-bio)

The new technical bulletin “Flow Cytometry protocol optimization”  by  Dr. Camilo Moncada, Ph.D. (Rockland Immunochemicals)  describes 5 ways to effectively improve flow cytometry results.

Content of the FCM technical note

    1. The right controls for flow cytometry
    2. Reducing noise from fluorescent signal
    3. Enhancing permeabilisation and fixation to improve detection of intracellular proteins
    4. Maintaining fluorescent signal at high intensity
    5. Staining dead cells and obtain meaningful data from viable cells

+ featured antibodies reagents optimized for FCM analysis allowing the detection of intracellular targets.

Access Rockland’s and tebu-bio’s flow cytometry tips now!

Technical tips icon by tebu-bio

 

Flow Cytometry protocol optimization”  by  Dr. Camilo Moncada, Ph.D. (Rockland Immunochemicals).

 

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