Dual secreted Alkaline Phosphatase and Luciferase detection in one sample

Secrete-Pair™ dual luminescence assay kit analyzes the activities of Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) as a dual-reporter system. This dual luminescence assay system includes all reagents needed for an optimal dual luminicent detection of GLuc and SEAP while allowing transfection normalization and side-by-side analysis using the same sample from the cell culture medium.

With Secrete-Pair™ dual luminescence assay kit (GeneCopoeia), samples can be easily obtained from cell culture medium without lysis of the cells.

Secreted GLuc and SEAP reporter proteins in live cell assays

Secreted GLuc signal comparison GeneCopoeia tebu-bio

Comparison of GLuc signal stability in different buffer systems from GeneCopoeia’s Secrete-Pair and a competitor Gaussia luciferase assay kit. Secrete-Pair buffer systems provide more stable GLuc signal than the competitor kit. Source: GeneCopoeia.

  • Dual-reporter detection
  • No cell lysis necessary
  • Real-time and fast data generation

Unique formation for robust and high sensitivity luminescence detection

  • Two buffer conditions are provided for GLuc assays:
    • Buffer GL-S with a stabilizer to overcome the quick decay of the GLuc signal.
    • Buffer GL-H with a specific formulation When higher sensitivity is required for detecting low expression of GLuc.
  • Optimized buffer for stable activity – 90+% of signal retained within the first 10 minutes and extends the half-life of light emission to approximately 30 minutes
  • Higher sensitivity – Ideal to detect low GLuc expression

Robust GLuc and SEAP data

  • Transfection normalization:
    • True cross-sample comparison
    • GLuc activities can be compared across samples free of the impact of transfection variation.
  • High-throughput compatible – high sample number compatible

Want to know more?

Find more about Secrete-Pair™ Dual  (GLuc and SEAP) Luminescence Assay Kits from GeneCopoeia (SPDA-D030 – 300 rxns and SPDA-D100 – 1,000 rxns)!

 

Trackbacks

  1. […] to the culture medium and only supernatant is used (keeping cells alive for later time points). SEAP levels are not sensitive to the miRNA machinery, thereby representing a readout of the transfection level. […]

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