Reactive oxygen species (ROS) play key roles in various intracellular processes and have been shown to be involved in many diseases (eg. carcinogenesis, inflammation…). Each of the ROS species is likely to have a specific role in living cells. Therefore, there is an emerging need for selectively detecting each species of ROS through conventional biochemical assays, but also in live cell imaging (see a previous post “Reactive Oxygen Species (ROS) and related assay kits“).
The detection of molecular events in living cells is booming. In this post, we look at 3 fluorescent probes that will undoubtedly count in the live-cell imaging landscape in 2017.
The Silicon Rhodamine-like (SiR) technology has significantly contributed to the recent development of DNA and cytoskeletal analysis by live cell imaging.
In 2014, two new Silicon Rhodamine-like (SiR) fluorescent probes were released for studying actin & tubulin by live cell imaging. SiR-Actin and SiR-Tubulin are fluorescent probes compatible with most microscopes (including super-resolution settings) that directly stain actin & tubulin without the need to transfect cells with vectors expressing fluorescently labeled Actin or Tubulin. The two original dyes were successfully followed by a new SiR-DNA probe in order to visualize DNA in living cells.
The existing SiR stains have a λabs of 652 nm and a λem of 674 nm to be used with the Cy5 filter (Fig 1).
However, the continuously growing number of researchers using these stains asked us whether stains with different biophysical properties would be made available. In other words, they were asking “is there another colour to allow for double staining e.g. of Actin and Tubulin in living cells?”
Super-resolution imaging techniques operate beyond the limit set by the diffraction of light, leading to new insights in cellular biology. Among these techniques, the direct stochastic optical reconstruction microscopy (dSTORM) approach is based on the use of blinking fluorescent dyes allowing spatial resolution of about 20 nm. This post is focusing on a new type of fluorescent dye compatible with both live-cell imaging and staining of fixed cells adapted to dSTORM. [Read more…]
Visualizing fixed cells and tissues only gives snapshots of cellular processes. To have a better insight into dynamic events ocurring in the cells or to vizualize interactions between various cellular components in real time (e.g., proteins, organelles, second messengers…), powerful microscopic approaches have been developed over the past decade. This post will review the recent live cell imaging probes developed by Goryo Chemicalsand available in Europe through tebu-bio.
In the autumn of 2014, we presented new stains to conduct live cell imaging of Actin and Tubulin, which I covered in my post 2 new Actin and Tubulin live-cell imaging stains – without transfection!
- No transfection
- No washing steps
- No toxic effects if used in the concentration range recommended
- Excellent brightness
- Far-red excitation & emission
- Deep tissue penetration and minimal background
- Multiple fluorescent stainings with other dyes possible
- Compatible with Superresolution microscopic techniques
Since then, Spirochrome has added another stain with the same benefits: SiR-DNA, a far-red, fluorogenic, cell permeable and highly specific probe for DNA (see Fig 1).
SLAS – High Content Screening Conference
From June 27th to June 29th, the SLAS – High Content Screening Conference took place in Dresden/Germany. The conference focussed on new essays and novel microscopy techniques.
Spirochrome and tebu-bio were selected to present a poster about the features of SiR-stains, which are excellent tools for phenotypic screening and high content screening (HCS) approaches.
If you are interested in learning more about these exciting live cell imaging tools, don’t hesitate to download our joint poster:
Any questions or comments? Please feel free to contact me with the form below!
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In a lot of experimental setups it would be eligible to be able to differentiate human ES and iPS cells from differentiated cells.
tebu-bio is pleased to announce their cooperation with Goryo Chemical, a company specialized in probes for Live Cell Imaging. Goryo Chemical recently launched the Kyoto probe 1 (KP-1) which exactly fulfills this need. [Read more…]