Human induced pluripotent stem (iPS) cells and cells differentiated from iPS cells have widely been used for in vivo models human disease progression. Jason Meyer, of Indiana University Purdue University Indianapolis, uses iPS cell-derived models to study retinogenesis and retinal disease. Two recent papers from his lab highlight the benefits of using Stemgent’s RNA reprogramming technology to enable robust differentiation of iPS cells to the retinal lineage (1, 2). RNA reprogramming technology was chosen in order for these studies to ensure that no vestiges of the reprogramming vectors were retained by the cells or integrated into the genome.
Staying at the forefront of innovative cellular reprogramming technologies, ReproCELL’s Stemgent RNA Reprogramming portfolio is being expanded to include a cellular reprogramming kit using self-replicative RNA (srRNA) to reprogram both human blood outgrowth endothelial progenitor cells (EPCs) and human fibroblasts to generate clinically relevant iPS cells. The srRNA reprogramming of EPCs into iPS cells is the first demonstrated commercial application of RNA for the cellular reprogramming of a blood-derived cell type. The non-viral/non-DNA nature of RNA reprogramming leads to clinically relevant iPS cell lines that are suitable for GMP-compliant research studies. [Read more…]
Reprogramming-qualified B18R Recombinant Protein, Carrier-free is of interest for cellular reprogramming and other applications requiring RNA-mediated gene delivery. B18R protein is a Vaccinia virus-encoded receptor with specificity for mouse, human, rabbit, pig, rat and cow Type 1 interferons and has potent neutralizing capability acting as a decoy receptor for Type 1 interferons.
- Stemfactor B18R Recombinant Protein, Carrier-free bioactivity is demonstrated by the neutralization of IFN-alpha or IFN-beta effects in an assay of IL-12 production from CD40L-activated monocytes.
- The biological function is validated by down-regulation of gene expression by qRT-PCR of TLR3, IFIT1, and OASI in response to B18R treatment on human fibroblasts to levels equivalent to non-transfected cells.
- The efficiency of mRNA reprogramming is tested by performing mRNA reprogramming with OKSML on neonatal foreskin fibroblasts and demonstrating greater than 0.5% iPSC colony formation.
Previously only available as part of the mRNA Reprogramming Kit or microRNA Booster Kit, Reprogramming-qualified B18R Recombinant Protein, Carrier-free is now available individually.
Human induced pluripotent stem (iPS) cells hold great promise for advancing our understanding of human biology and medicinal research. It has been shown that ectopic expression of just a few key transcription factors in somatic cells can induce an embryonic stem cell phenotype hallmarked by the ability to differentiate into any cell of the three basic germ layers (1,2). [Read more…]
Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular models for personalized therapies and regenerative medicine.
mRNAs are expression factors that mimic fully processed mRNA. Being the substrate for translation by ribosomes, mRNA expression factors are often preferred over viral vectors for cell reprogramming and iPS cell generation because of the absent risk of integration into the genome. Such RNA-induced pluripotent stem cells (RiPSCs described in 2010 by Warren et al.) are becoming more and more popular. 3 reasons might illustrate RiPSCs’ attractivty.
In 2010, a method for integration-free reprogramming by transfecting modified mRNA reprogramming factors was published (1, 2). The use of mRNA to induce reprogramming of somatic cells overcomes the inherent problems incurred by introducing viral vectors and/or integrating DNA to target cells. mRNA reprogramming factors are titratable and controllable with regards to the ratios, concentrations, and timing of factor expression. The mRNA reprogramming system also poses none of the biosafety risks surrounding viral and DNA-based systems. [Read more…]