Methods commonly used for the characterization of iPS or ES cells are based on specific antibody staining. Nevertheless, undifferentiated stem cell populations can also be characterized by high levels of both Alkaline Phosphatase (ALP) and Aldehyde Dehydrogenase (ALDH) activities. [Read more…]
Alzheimer’s Disease (AD) is a type of dementia which induces progressive and non-reversible brain disorders (memory and cognitive loss, thinking and behavior changes…). AD ultimately ends in severe mental dysfunction due to neuronal death and breakdown of neural connections. The majority of people with AD are 65+ (late-onset AD). Even if age is one of the greatest risk factors, >5% of AD patients are in their 40s or 50s (early onset AD) making genes and heredityother important AD risk factors (1-4). [Read more…]
Mitochondria and their functional status provide an early indication of cellular toxicity and thus have emerged as a critical target in drug discovery and toxicity profiling. Apoptosis, necrosis and slow degenerative disease all exhibit changes in the electrochemical gradient across the mitochondrial membranes, ΔΨm, which give rise to the creation of the electronic potential necessary for ATP production. [Read more…]
The transition from non-invasive phenotype to invasive phenotype of tumor cells marks the switch from a benign tumor to a more malignant form of cancer. Understanding the mechanisms underlying this hallmark event, which enables tumor cells to invade through Extracellular matrix, is critical for discovering pathways and new targets to develop anti-metastatic strategies. The discovery of anti-metastatic agents that inhibit cancer cell motility has been hindered by a dearth of cell motility assays that are compatible with high–throughput screening. [Read more…]
The Notch signaling pathway plays a vital role in the regulation of asymmetric cell division, cell fate specification and proliferation. Mammals possess 4 different Notch receptors, referred to as Notch1, 2, 3, and 4. The Notch receptor is a hetero-oligomer transmembrane protein that comprises a large extracellular domain, a single-pass transmembrane span, and a small intracellular region. [Read more…]
An interesting poster has recently been published by XenoTech: “Selection of Human Liver S9 and Cytosol Fractions for Evaluating Clearance by Aldehyde Oxidase (AO): The Impact of Low Versus High AO Activity Lots” by Phyllis Yerino, Clayton Otwell, Zell Woodworth and David B Buckley (XenoTech, LLC, Lenexa, KS, USA). [Read more…]
Pr. Ruo-Pan Huang’s team has just published a review detailing the interactions between cancer and immune cells.
This overview describes the key cytokine signals, cell–cell signal networks in the tumor microenvironment during cancer diseases. It also demonstrates the power of antibody arrays and multiplex immunoassays as unique research tools to decipher cytokine networks during cancer and inflammation processes.
Brett Burkholder et al. “Tumor-induced perturbations of cytokines and immune cell networks” (2014) BBA – Reviews on Cancer, Vol. 1845 no. 2, 182–201. DOI http://dx.doi.org/10.1016/j.bbcan.2014.01.004
Study of living tissues has gained wide spread application in microscopy with the advent of fluorescent dyes and inverted microscopes dedicated to their viewing. A critical component is maintaining physiological conditions in media formulations, including pH and osmolarity, without contributing to background fluorescence. Most cells are grown in media formulations that use bicarbonate buffers, requiring the continuous presence of CO2 to maintain physiological pH. Just 15 minutes outside a CO2 incubator can change the pH from 7.4 to 8.1, and to 8.7 after an hour. [Read more…]
Cell reprogramming of Mouse and Human somatic cells to induced Pluripotent Stem Cells (iPSC) have opened outstanding new opportunities for biomedical research. iPSC are now becoming unique cellular models for personalized therapies and regenerative medicine.