The Blood-Brain Barrier (BBB) affects the development of drugs for all pathologies (brain exposure to drugs, side effects…). In the early phases of drug development, there is a strong need for stable and fully characterized human brain endothelial cellular models that might mimic the molecular and cellular interactions between the blood and the brain in vitro.
Let’s take a look at a brain endothelial cell line with proven applications in drug discovery: the hCMEC/D3 Human Cerebral Microvascular Endothelial Cell Line.
If you’re studying human pulmonary function and pathophysiology, you need access to validated, highly characterised, human airway cellular models. A large collection of these cells is available for applications in asthma, inhalation toxicology and pulmonary inflammatory response. Let’s take a look at a selection of different pulmonary cell types I would advise, to boost your in vitro research in this area.
Human cells are critical raw materials for research and manufacturing of cell therapy products. However, access to freshly procured cells can be limited, creating a crucial need for a suitable alternative to fresh cells that are viable and functional — especially when transporting materials globally.
At tebu-bio, we chose HemaCare to investigate the viability and functionality of lymphocytes, both fresh and cryopreserved, from leukopaks (leukapheresis collections) procured within their FDA registered cGMP donor collection facility. [Read more…]
Thousands of researchers spend time pampering their primary cells and cell lines every day, with the aim of defining their optimal cell culture conditions. Researchers are in effect trying to control numerous cellular parameters (oxygene levels, fresh media supply, incubation temperature…) and monitor their “viability” on a regular basis. But what about the internal cellular temperature? Have you ever considered this biological parameter to monitor the “wellness” of your cultivated cells? In this post, let’s take a look at the characteristics of a new, cell permeable, fluorescent thermometer for living cells.
Cord blood might be a much-needed solution.
Few diseases stump researchers as much as Alzheimer’s disease. Quite simply, nothing has worked beyond some symptomatic relief. On top of that, scientists are still uncertain about what exactly causes the disease. For example, are the characteristic beta-amyloid plaques in the brain a cause of Alzheimer’s disease — or a consequence? Any promising results are welcome to a community used to setbacks. [Read more…]
Interferons (IFNs) are low molecular weight proteins that belong to the class of glycoproteins known as cytokines. IFNs are part of the non-specific immune system and are an important first line of defense against viral infections. They are released by host cells in response to the presence of pathogens such as viruses, bacteria, parasites or tumor cells.
IFNs have other functions as well: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection. [Read more…]
Staying at the forefront of innovative cellular reprogramming technologies, ReproCELL’s Stemgent RNA Reprogramming portfolio is being expanded to include a cellular reprogramming kit using self-replicative RNA (srRNA) to reprogram both human blood outgrowth endothelial progenitor cells (EPCs) and human fibroblasts to generate clinically relevant iPS cells. The srRNA reprogramming of EPCs into iPS cells is the first demonstrated commercial application of RNA for the cellular reprogramming of a blood-derived cell type. The non-viral/non-DNA nature of RNA reprogramming leads to clinically relevant iPS cell lines that are suitable for GMP-compliant research studies. [Read more…]
Newly added to the Stemolecule portfolio are three small molecules which support a new approach for the derivation and maintenance of naïve human pluripotent stem cells. These three newly identified small molecules, WH-4-023, SB590885 and IM-12, are all kinase inhibitors.
Today, following our series on Tumour Microenvironment (TME), and leading on from three recent publications, let’s discuss Cancer Stem Cells (CSCs).
CSCs are part of the TME, as they reside in niches where they can survive from immune surveillance, maintain plasticity and facilitate cancer metastasis (1). They can also circulate around the body. Which makes them so interesting. Their worse effect is that they can expand cancer to other organs in the body, thus helping metastasic. The good thing is that their early detection is a very powerful biomarker for cancer, allowing an early treatment. [Read more…]