Dojindo just completed its offer in the field of mitochondrial research with the release of a unique fluorescent dye for live cell imaging. This post introduces this new MitoPeDPP mitochondrial probe.
Multicolour fluorochrome reagents have spurred researchers’ capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let’s take at look at the advantages of 5 optimized combinations of mixed fluorochromes you should consider for your next immunophenotyping assays by multicolour flow cytometry.
In this post, I’d like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation, and glycylation. This is inspired by contribution provided by Cytoskeleton Inc., who are experts in this domain.
The unprecedented increase in antibiotic-resistant pathogens and lack of new antibiotic development highlights the need for new anti-microbial drugs active against novel targets such as bacterial cell division proteins. Recently, FtsZ (Filamenting temperature sensitive mutant Z) has become the focus of antibiotic research as a novel target for new anti-microbials (1, 2). [Read more…]
New research suggests that dendritic cells produce and release CTLA-4, which typically inhibits anticancer responses.
Cancer immunotherapy strategies have made it increasingly evident that the immune system plays an integral role in managing and destroying cancer. Nevertheless, many mechanisms of immune suppression exist that may inhibit antitumour immunity. Recently, strategies that implement antibodies directed against negative immunologic regulators have demonstrated significant success. Cytotoxic T-lymphocyte-associate protein-4 (CTLA-4) was the first immunologic checkpoint to be clinically targeted, by the cancer immunotherapeutic ipilimumab, an FDA-approved drug to treat melanoma. After T-cell activation, CTLA-4 is upregulated on the cell surface where it functions to downregulate T cell function. Ipilimumab binds to CTLA-4 on T cells, which blocks the inhibitory signals and enhances anti-cancer immune responses.
Super-resolution imaging techniques operate beyond the limit set by the diffraction of light, leading to new insights in cellular biology. Among these techniques, the direct stochastic optical reconstruction microscopy (dSTORM) approach is based on the use of blinking fluorescent dyes allowing spatial resolution of about 20 nm. This post is focusing on a new type of fluorescent dye compatible with both live-cell imaging and staining of fixed cells adapted to dSTORM. [Read more…]
In the autumn of 2014, we presented new stains to conduct live cell imaging of Actin and Tubulin, which I covered in my post 2 new Actin and Tubulin live-cell imaging stains – without transfection!
- No transfection
- No washing steps
- No toxic effects if used in the concentration range recommended
- Excellent brightness
- Far-red excitation & emission
- Deep tissue penetration and minimal background
- Multiple fluorescent stainings with other dyes possible
- Compatible with Superresolution microscopic techniques
Since then, Spirochrome has added another stain with the same benefits: SiR-DNA, a far-red, fluorogenic, cell permeable and highly specific probe for DNA (see Fig 1).
SLAS – High Content Screening Conference
From June 27th to June 29th, the SLAS – High Content Screening Conference took place in Dresden/Germany. The conference focussed on new essays and novel microscopy techniques.
Spirochrome and tebu-bio were selected to present a poster about the features of SiR-stains, which are excellent tools for phenotypic screening and high content screening (HCS) approaches.
If you are interested in learning more about these exciting live cell imaging tools, don’t hesitate to download our joint poster:
Any questions or comments? Please feel free to contact me with the form below!
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Singlet Oxygen (1O2) fluorescent detection in living cells was limited by the lack of availability of cell membrane permeable dyes. The silicon-based far-red fluorescent probe (Si-DMA) has been especially designed to monitor singlet oxygen in real-time and in living cells. Si-DMA is now commercially available for ROS studies by live-cell imaging.
The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious and based on the use of harmful chemicals. Today, easy-to-use and straightforward methods with colorimetric read-outs are used daily. [Read more…]
In a lot of experimental setups it would be eligible to be able to differentiate human ES and iPS cells from differentiated cells.
tebu-bio is pleased to announce their cooperation with Goryo Chemical, a company specialized in probes for Live Cell Imaging. Goryo Chemical recently launched the Kyoto probe 1 (KP-1) which exactly fulfills this need. [Read more…]