The CRISPR-CAS9 system may well have opened Pandora’s box, but it is also definitely the cornucopia of genome editing.
We can do what we want in the genome: settle a mutation, correct a mutation, insert a fluorescent tag to a protein, add an exogenous gene, delete an endogenous function, suppress a cis-regulatory region, add a reporter…. I might just not have enough imagination!
The main challenge is to define a good strategy, taking in account the specifics of the project and being aware of the corresponding limitations. [Read more…]