Small molecules known to modulate biological activies of the K-Ras oncogene are predominantly of synthetic origin (ex. Salirasib). Nevetheless, various publications have recently demontrated the biological potential of microbial metabolites in KRAS studies. Here’s a good opportunity to briefly review a few of these compounds.
Singlet Oxygen (1O2) fluorescent detection in living cells was limited by the lack of availability of cell membrane permeable dyes. The silicon-based far-red fluorescent probe (Si-DMA) has been especially designed to monitor singlet oxygen in real-time and in living cells. Si-DMA is now commercially available for ROS studies by live-cell imaging.
The role of the interactions between tumor cells and their microenvironment (TME) is now well-established in various stages of cancer development. Cytokines, produced by both the cancer and the immune cells, actively contribute to these critical connections (see the previous posts related to the crosstalk between cancer and immune cells in relation to tumor escape, immunoediting and immunosurveillance).
In a recent review published in BBA, RayBiotech‘s team highlights the “cytokine signatures and dynamics” seen during cancer development. They also demonstrate the necessity of designing relevant multiplex immunoassays for the profiling and the quantification of cytokines in biomarker and anti-cancer drug discovery programs.
Valerie Sloane Jones, Ren-Yu Huang, Li-Pai Chen, Zhe-Sheng Chen, Liwu Fu, Ruo-Pan Huang “Cytokines in cancer drug resistance: Cues to new therapeutic strategies” (2016) Biochimica et Biophysica Acta, Volume 1865, Issue 2, 255–265. DOI:10.1016/j.bbcan.2016.03.005.
Download the publication written by RayBiotech, the leader in planar Antibody Arrays.
Removal of contaminating bacterial chromosomal DNA in plasmid preparations (but also cosmid, BAC…) is crucial in today’s precise molecular biology experiments. In this post, a cost-effective but powerful enzyme is described for molecular biologists looking for genomic DNA-free preps.
Accurate monitoring of genome transcription activities is of crucial interest for deciphering gene expression and for a better understanding of RNA biology. Over the past years, various experimental methods for RNA Pol II mapping density across the whole genome have been designed. Here, I’d like to offer a brief introduction to the human Native Elongating Transcript-Sequencing (NET-Seq) method.
There is a high demand for selective fluorescent probes for the imaging of particular organelles and biomarkers in living cells. This is particularly the case for mitochondrial research. In this post, we will focus on another type of powerful research tools (ie. primary antibodies) that have been proven to be very useful for studying mitochondria. The 10 antibodies highlighted here have been successfully used by our clients for immunoblotting of mitochondrial proteins and are all covered by tebu-bio’s Satisfaction Guaranteed.
The Wnt cell signalling pathway is a high-potential therapeutical target in cancer research. It’s deeply linked to cellular fate through modulating cell proliferation, mobility, interaction, and polarity. Wnt is not only a key player in the embryonic development but also in the homeostasis, and remodeling of adult tissues (heart, bone, neural tube, angiogenesis…) via somatic stem cell modulations and stem cell self-renewal. Abnormal Wnt signaling events are seen in various types of human carcinomas and in a variety of other cancer types.
The accumulation of insoluble misfolded phosphorylated tau protein in neurofibrillary tangles is one of the hallmarks of tau pathologies. Preformed fibrils generated from recombinant tau fragments have been shown to induce tau fibrilization and spreading of the pathology to interconnected brain regions in an in vivo tauopathy model. (1) Abnormal tau degradation and aggregation have been correlated with cognitive decline in Alzheimer’s disease (AD). [Read more…]
The year 2015 was full of significant publications in the field of ophthalmology. Here’s a snapshot of 2 “proof-of-concept” studies suggesting that “Sterols” could be promising approaches to treat cataract.
The anti-cancer property of the Vitamin K3 (Vitk3) was again recently studied by Dr Meng-Er Huang’s team (Curie Institute, CNRS UMR3348, Paris).
By using in vitro pro-oxydative cellular models, Dr Huang and collaborators observed a cancer-selective cytotoxicity of vitk3 in Peroxyredoxin 1 (PRDX1 aka PRX1)-deficient cancer cell lines.
To further investigate the underlying molecular mechanisms, the authors used a stably PRX1-depleted HeLa cell line (PRX1-) (vs. Control (PRX1+)) under various experimental conditions. They notably treated PRX1- and PRX1+ cell lines by vitk3 and 6 other known anticancer molecules targeting different cellular events (microtubule (vinblastine & taxol); DNA (doxorubicin & daunorubicin); DNA transcription (actinomycin D) and cell cycle (5-FU)). [Read more…]