There is an increasing demand for new cholesterol-lowering therapies in addition to existing treatments (e.g. targeting statins). The pharmacological inhibition of PCSK9-mediated LDLR degradation is becoming very attractive for treating cholesterol-related diseases. But which reliable in vitro assays are available to effectively study “PCSK9-LDLR” interactions ?
In this post, I’d like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation, and glycylation. This is inspired by contribution provided by Cytoskeleton Inc., who are experts in this domain.
Over the past months, we have released a number of posts introducing inhibitor screening assays intended to investigate immuno checkpoint protein interactions, such as PD-1/PD-L1/PD-L2; B7-1/CD28, B7-1/CTLA4; BLTA/HVEM, CD47/SIRPα; GITR/GITRL and many others – for an overview, you might like to read 9 pathway-specific screening assays in Immunotherapy.
Today, I’d like to concentrate on the TIGIT/CD226 pathway, because it acts through a novel mechanism to regulate CD8+ T cell functions within the tumour microenvironment. [Read more…]
The unprecedented increase in antibiotic-resistant pathogens and lack of new antibiotic development highlights the need for new anti-microbial drugs active against novel targets such as bacterial cell division proteins. Recently, FtsZ (Filamenting temperature sensitive mutant Z) has become the focus of antibiotic research as a novel target for new anti-microbials (1, 2). [Read more…]
Polyethylene Glycols (aka. PEGs) are biologically inert, non-immunogenic, and hydrophilic molecules. These properties make them very attractive for research and drug discovery applications (ex. Antibody-Drug Conjugation, imaging, enzymatic detection). The aim of this post is to introduce recent developments in the field of PEG technology provided by BroadPharm.
Super-resolution imaging techniques operate beyond the limit set by the diffraction of light, leading to new insights in cellular biology. Among these techniques, the direct stochastic optical reconstruction microscopy (dSTORM) approach is based on the use of blinking fluorescent dyes allowing spatial resolution of about 20 nm. This post is focusing on a new type of fluorescent dye compatible with both live-cell imaging and staining of fixed cells adapted to dSTORM. [Read more…]
Visualizing fixed cells and tissues only gives snapshots of cellular processes. To have a better insight into dynamic events ocurring in the cells or to vizualize interactions between various cellular components in real time (e.g., proteins, organelles, second messengers…), powerful microscopic approaches have been developed over the past decade. This post will review the recent live cell imaging probes developed by Goryo Chemicalsand available in Europe through tebu-bio.
In the autumn of 2014, we presented new stains to conduct live cell imaging of Actin and Tubulin, which I covered in my post 2 new Actin and Tubulin live-cell imaging stains – without transfection!
- No transfection
- No washing steps
- No toxic effects if used in the concentration range recommended
- Excellent brightness
- Far-red excitation & emission
- Deep tissue penetration and minimal background
- Multiple fluorescent stainings with other dyes possible
- Compatible with Superresolution microscopic techniques
Since then, Spirochrome has added another stain with the same benefits: SiR-DNA, a far-red, fluorogenic, cell permeable and highly specific probe for DNA (see Fig 1).
SLAS – High Content Screening Conference
From June 27th to June 29th, the SLAS – High Content Screening Conference took place in Dresden/Germany. The conference focussed on new essays and novel microscopy techniques.
Spirochrome and tebu-bio were selected to present a poster about the features of SiR-stains, which are excellent tools for phenotypic screening and high content screening (HCS) approaches.
If you are interested in learning more about these exciting live cell imaging tools, don’t hesitate to download our joint poster:
Any questions or comments? Please feel free to contact me with the form below!
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The monitoring of ECM components during experimental approaches requires robust and user-friendly in vitro assays. In the past experimental methods (e.g. soluble collagen measurement) were laborious and based on the use of harmful chemicals. Today, easy-to-use and straightforward methods with colorimetric read-outs are used daily. [Read more…]
In a lot of experimental setups it would be eligible to be able to differentiate human ES and iPS cells from differentiated cells.
tebu-bio is pleased to announce their cooperation with Goryo Chemical, a company specialized in probes for Live Cell Imaging. Goryo Chemical recently launched the Kyoto probe 1 (KP-1) which exactly fulfills this need. [Read more…]