Multicolour fluorochrome reagents have spurred researchers’ capacity to analyze cell populations and to isolate cell subsets by flow cytometry. In this post, let’s take at look at the advantages of 5 optimized combinations of mixed fluorochromes you should consider for your next immunophenotyping assays by multicolour flow cytometry.
In this post, I’d like to take a look at the current understanding of tubulin PTMs, that include tyrosination/detyrosination, Δ2-tubulin formation, acetylation, phosphorylation, ubiquitination, glutamylation, and glycylation. This is inspired by contribution provided by Cytoskeleton Inc., who are experts in this domain.
Reactive Oxygen Species (ROS) are reactive molecules and free radicals derived from molecular oxygen involved in cellular homeostasis. An excess of ROS production (e.g. exposure to environmental stress such as UV or heat exposure) causes significant damage to cell structures. In this post, let’s review the research tools for studying this process also known as Oxidative […]
Over the past months, we have released a number of posts introducing inhibitor screening assays intended to investigate immuno checkpoint protein interactions, such as PD-1/PD-L1/PD-L2; B7-1/CD28, B7-1/CTLA4; BLTA/HVEM, CD47/SIRPα; GITR/GITRL and many others – for an overview, you might like to read 9 pathway-specific screening assays in Immunotherapy. Today, I’d like to concentrate on the […]
Today, I’d like to invite you to take a look at a highly efficient and useful kit, which brings together all the required components you need in a complete system for culturing and transfecting human pluripotent stem cells for gene editing. The PluriQ™ G9™ Gene Editing System system includes the G9™ Maintenance Medium and G9™ […]
Sequestosome-1 is a protein encoded by the SQSTM1 gene in humans, that is included in the autophagosome, and targets other proteins that bind to it for selective autophagy. Autophagy was first described in the 60s, and it presents clear differences vs apoptosis. While apoptosis is a mechanism that kills the cells (apoptosis = self-killing), autophagy […]
The unprecedented increase in antibiotic-resistant pathogens and lack of new antibiotic development highlights the need for new anti-microbial drugs active against novel targets such as bacterial cell division proteins. Recently, FtsZ (Filamenting temperature sensitive mutant Z) has become the focus of antibiotic research as a novel target for new anti-microbials (1, 2).
I have to admit that I never received the Fields Medal in Mathematics. Therefore, I won’t be able to develop this equation and prove that I’m right. However, what I can prove, is that in cell culture, 2 = 5. How is this possible?
New research suggests that dendritic cells produce and release CTLA-4, which typically inhibits anticancer responses. Cancer immunotherapy strategies have made it increasingly evident that the immune system plays an integral role in managing and destroying cancer. Nevertheless, many mechanisms of immune suppression exist that may inhibit antitumour immunity. Recently, strategies that implement antibodies directed against […]
All cell types are in contact with the ExtraCellular Matrix (ECM), a complex and dynamic network of macromolecules with different physical and biochemical properties. The ECM plays an essential rule in structural support, adhesion, movement and cell regulation. Its components are frequently monitored, which requires robust and user-friendly kits. In this post, I invite you to […]